| Customization: | Available |
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| Environmental Protection: | Yes |
| Certification: | REACH |
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1. Background
Colistn is a polypeptide antibiotic, which is commonly applied in curing sensitive organism infection and promoting growth of pup and poultry. It is employed as medicine feed additives and inhibitor of Gram-negative bacteria such as bacillus pyocyaneus and Escherichia coli. Colistn is excreted by kidney, and it can cause kidney epithclium denaturalization.
The common approach to detect colistn is microbiology inhibition assay. Due tothe big molar weight, strong polarity and thermal instability, it is inappropriate to detect colistn with GC, thus the sensitivity and detection limit is restricted. Enzyme immunoassay is more precise and sensitive, and simpler operating, and can considerably minimized work intensity.
2. Principle
This kit is based on indirect-competitive ELISA technology. The microtiter wells are coated with coupling antigen. Colistn residue in the sample competes with the antigen coated on the microtiter plate for the antibody. After the addition of enzyme conjugate, TMB substrate is used to show the color. Absorbance of the sample is negatively related to the colistn residue in it, after comparing with the Standard Curve, multiplied by the dilution multiple, Colistn residue quantity in the sample can be calculated.
3. Applications
This kit can be used in quantitative and qualitative analysis of colistn residue in tissue, milk.
4.Detection limit
5ppb
Certificate
ISO9001: 2008 & ISO13485: 2003;
CNAS (China National Accreditation Service for Conformity Assessment);
CQC (China Quauty Certification Center);
CE (Communate Europpene).
Awards
High-Tech Enterprise;
Key High-Tech Enterprise of China Torch Program;
Beijing Engineering Research Center;
Industrialization Demonstration Project of China Torch Program;
Beijing Science and Technology Award;
Tianjin Science and Technology Award;
Shandong Science and Technology Award;
Stuff
Kwinbon currently has more than 500 employees, including 18 doctors, 92 masters and 24 senior engineers. And also 46% of the employees are working on R&D.
Stock Market
NEEQ (National Equities Exchange and Quotations); Stock Code: 835044.
Facility
GMP (Good Manufacturing Practice) factory and SPF animal house. The cleanliness of producing department can reach above level 105.
Instruments
HPLC, GC, LC-MS/MS for test result calibration, which are expected to provide better quality control of our test products.
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