Ochratoxin a Residue Test Strip for Cereal

• Express: DHL FedEx
• Samples: Rice Peanut Feed
• Recovery Rate: ≥80%
• Transport Package: Ice Bag with Foam Box
• Specification: 50T
• Trademark: kwinbon
• Model NO.: KH00402Z
• Origin: China
• HS Code: 38229000
• Production Capacity: 5000000000 PCS

Product Description

Ochratoxin A Immunoaffinity columns

1. Principle

The ochratoxin A immunoaffinity column is based on antigen- antibody-reaction technology, which makes the test highly specific, sensitive, rapid and simple to perform.
The columns contain a gel suspension of monoclonal antibody specific to the toxin of interest. Following extraction of the toxin the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxin is then released from the column following elution with solvent. The final elution solution is for assay.
   It can reduce efficiently the sample impurity interference and improve the accuracy of test if it is combined with the HPLC, fluorescence and immunoassay.

1. Sample

It can test the maize, soybean, peanut, wheat, barley, rice and finished feed.

1. Required equipment

----Homogenizer
----Vortex mixer
----Sample bottle
----Volumetric flask: 10ml, 100ml
----Qualitative filter paper/Centrifuge
----Analytical balance (inductance: 0.01g)
----Graduated pipette: 10ml
----Injector: 10ml
----Volumetric flask: 250ml
----Rubber pipette bulb
----Micropipette: 100-1000ul

1. Reagent

----Methanol (AR)
----Deionized water

1. Sample preparations

----Homogenize the sample.
----Take 3.0±0.05g sample into sample bottle. Add 6ml of methanol and vortex for 2min or shake for 10min with table concentrator.
----Centrifuge: 5min/3000g at least/room temperature (20-25ºC). (If no centrifuge, it can also stand the solution and take 10ml of the supernatant to filter)
----Take 2ml (1g sample) of supernatant after centrifuge or filter and add 8ml deionized water. Mix completely and be for assay.

1. Assay process

----Take out the columns in the 2-8ºC/35.6-46.4ºF and recover to room temperature (20-25ºC/68-77ºF).
----Connect the columns to the 10ml injector by the joint (picture 1). 
----Pipette 5ml (0.5g sample) of prepared solution into the injector→→use the injector to control the flow rate of the liquid to 1-2 seconds per drop through the column. 
---- Wash the column with 5ml deionized water for twice →→use the injector to control the flow rate of the liquid to 0.5-1 seconds per drop through the column. Finally drain the liquid in the column.

• ----Elute with 1ml methanol→→use the injector to control the flow rate of

the liquid to 2-3 seconds per drop through the column. Collect all the eluent and be for assay.
Notice:
----Peristaltic pump can also be used to control the flow rate to through the column.

• ----For a mass of test, using the pump flow operation can reduce the working time and intension.

7. Sample dilute ratio and recovery rate

Sample dilute ratio: 2 time

Recovery rate: ≥80%

8.Storage

Storage at 2-8ºC/35.6-46.4ºF. The period of validity is 12months.