Milkguard β -Lactams & Cephalosporins & Ceftiofur&Tetracyclines&Florfenicol Test Kit Kb26601y

Product Description

Product Description

MilkGuard
β-Lactams & Cephalosporins & Ceftiofur&Tetracyclines&Florfenicol Test Kit

1. Principle

This kit is based on the specific reaction of antibody-antigen and immunochromatography. β-Lactams, cephalosporins , tetracyclines and flufenicol antibiotics in the sample compete for the antibody with the antigen coated on the membrane of the test strip. Then after a color reaction, the result can be observed.

1. Applications

This kit is used for rapid qualitative analysis of β-lactams, cephalosporins , tetracyclines and florfenicol in raw milk sample.

1. Limit of Detection (LOD)

	MRL(ug/L)	LOD(ug/L)		MRL(ug/L)	LOD(ug/L)
Florfenicol (T1)		0.08-0.1	Thiamphenicol (T1)		0.2-0.3
Beta-lactams (T2)			beta-lactams ( T2 )
Penicillin G	4	1	Nafcillin	30	15-20
Ampicillin	10	3-4	Cefoperazone	-	2-3
Amoxicillin	10	3-4	Ceftriaxone	-	20-30
Oxacillin	30	5-6	Ceftiofur(low LOD)	100	60-80
Cloxacillin	30	5-6	Cefalonium	20	2
Dicloxacillin	30	3-5	Cefquinome	20	15-20
Cefacetrile	-	50-70	Cefepime	--	10-15
Cephalothin	-	25-30	Cephapirin	--	15-20
Desfuroyl ceftiofur		20-30	Cefazolin		70-90
Ceftiofur and metabolite desacetylcephapirin		40-50ppb
Cephalosporins	MRL(ug/L)	LOD(ug/L)	Tetracyclines(T5)	MRL(ug/L)	LOD(ug/L)
Cefalexin (T3)	100	10-15	Tetracycline	100	15-20
Cefradine(T3)	-	10-15	Oxytetracycline	100	15-20
Cefadroxil(T3)	-	10-15	Doxycycline	100	10-15
Ceftiofur (T4, high LOD)	---	20-25	Chlortetracycline	100	15-20

1. Kit components

8-well and 8 test strip in one bottle. 12 bottles / kit.	Kit insert
Microwell holder	Plastic pipette, 96pcs

** Use one strip for one sample, the rest strips can be stored in the bottle for future use.

1. Instrument needed but not provided

Metal incubator(suggested product: Kwinbon Mini-T4)

1. Assay operations

1. Read the instructions carefully before experiment. Bring the test kit and samples to room temperature. Milk samples should be fully liquid without any agglomeration or deposition.
2. Take bottles needed from the kit package, take out required wells and strips, and make proper marks. Please use these test strips within 1h. Seal the cap of the bottles. The rest strips can be stored for future use.
3. Preheat the metal incubator to 45ºC.
4. Place the mircrowells in the metal incubator, Take 200ul of the test samples into the wells, then repeatedly absorb and drop for 5 times to mix the sample with the reagent in the wells completely. The mixture should be pink, and then start the timer.
5. Incubate for 3min in the metal incubator (45ºC); insert the test strips into the wells with the "Sample pad" end fully dipped in to the mixture.
6. Incubate for 7min in the metal incubator(45ºC) again. Take out the strip; judge the result according to Part 7. (Read the result within 2min)

7.Results
There are 6 lines in the strip, Control line, Tetracylcines Line, Ceftiofur Line, Cephalosporins Line(cefalexin,cefadroxil,cefradine) , ßeta-lactams(including low LOD ceftiofur) Line and Florfenicol& Thiamphenicol line, which are briefly used as "C","T5 ","T4" "T3", "T2" and "T1". The test results will depend on the color of these lines. The following diagram describes the result identification.
Negative (-): Line T and Line C are both red, color of Line T is stronger than or similar to Line C, indicating the corresponding residue in sample is less than LOD of the kit.
Positive (+): Line C is red, color of Line T is weaker than Line C or Line C is red, Line T is colorless, indicating the corresponding residue in sample is higher than LOD of the kit.
Invalid: Line C has no color, which indicates the strips are invalid. In this case, please read the instructions again, and redo the assay with new strip.

8.Specificity
The product shows negative at the level of 500μg/L of melamine, chloramphenicol, enrofloxacin, sulfadimidin etc.
9.Storage
2-8ºC in cool dark place, do not freeze. The kit will be valid in 12 months. The lot number and expired date are printed on the package.
10.Notice for operations

1. 1. Please do the assay following the instruction, do not touch the membrane of the strip.
   2. Lab temperature should be 18-30ºC.
   3. Please seal the bottle after taking out required strips.
   4. This strip is used for only once; please do not use it repeatedly.
   5. This kit is only for screening test, positive result should be further confirmed with other method.

 

Detailed Photos

 

Company Profile

Stuff

Kwinbon currently has more than 500 employees, including 18 doctors, 92 masters and 24 senior engineers. And also 46% of the employees are working on R&D.

Stock Market

NEEQ (National Equities Exchange and Quotations); Stock Code: 835044.

Facility

GMP (Good Manufacturing Practice) factory and SPF animal house. The cleanliness of producing department can reach above level 105.

Instruments

HPLC, GC, LC-MS/MS for test result calibration, which are expected to provide better quality control of our test products.
 

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