Product Description
MilkGuard B+T Combo Test
β-Lactams & Tetracyclines Combo Test Kit
- Principle
This kit is based on the specific reaction of antibody-antigen and immunochromatography. β-lactams and tetracyclines antibiotics in the sample compete for the antibody with the antigen coated on the membrane of the test strip. Then after a color reaction, the result can be observed.
- Applications
This kit is used for rapid qualitative analysis of β-lactams and tetracyclines in milk sample.
- Detection Limit (LOD)
β-lactams |
MRL(μg/L) |
LOD(μg/L) |
Cephalosporins |
MRL(μg/L) |
LOD(μg/L) |
penicillin G |
4 |
1.5-2 |
cefquinome |
20 |
10-12 |
ampicillin |
4 |
3-4 |
cefacetrile |
125 |
20-25 |
amoxicillin |
4 |
3-4 |
cefalonium |
20 |
6-8 |
oxacillin |
30 |
6-8 |
cefazolin |
50 |
30-40 |
cloxacillin |
30 |
6-8 |
cefoperazone |
50 |
4-6 |
dicloxacillin |
30 |
6-8 |
cephapirin |
60 |
10-15 |
nafcillin |
30 |
10-20 |
ceftiofur |
100 |
90-100 |
Cefalexin |
100 |
800 |
Ceftriaxone |
-- |
100 |
Tetracyclines |
MRL(μg/L) |
LOD(μg/L) |
Tetracyclines |
MRL(μg/L) |
LOD(μg/L) |
tetracycline |
100 |
30-40 |
doxycycline |
100 |
30-40 |
oxytetracycline |
100 |
30-40 |
chlortetracycline |
100 |
30-40 |
- Kit components
- 8-well strip and 8 test strip in one bottle. 12 bottles / kit.
|
- Kit insert
|
- microwell holder
|
- Plastic pipette, 96pcs
|
** Use one strip for one sample, the rest strips can be stored in the bottle for future use.
- Assay operations
- Read the instructions carefully before experiment. Bring the test kit and samples to room temperature. Milk samples should be fully liquid without any agglomeration or deposition.
- Take bottles needed from the kit package, take out required wells and strips, and make proper marks. Please use these test strips within 1h. Seal the cap of the bottles. The rest strips can be stored for future use.
- Take 200ul of the test samples into the wells, then repeatedly absorb and drop for 3 times to mix the sample with the reagent in the wells completely. The mixture should be pink, and then start the timer.
- Incubate for 3min at room temperature (25oC); insert the test strips into the wells with the "MAX" end fully dipped in to the mixture.
- Incubate for 3min at room temperature again. Take out the strip; judge the result according to Part 6.
- Results
There are 3 lines in the strip, Control line, Beta-lactams Line and Tetracylcines Line, which are briefly used as "C", "B" and "T". The test results will depend on the color of these lines. The following diagram describes the result identification.
Negative: Control line, B Line and T Line are all red,and color of line T,B all stronger or the same as color of C line .
Beta-lactams Positive: Control Line is red, B Line is weaker color than line C;
Tetracyclines Positive: Control Line is red, T Line is weaker color than line C;
Beta-lactams and Tetracyclines Positive: Control Line is red; B Line and T Line are all with weaker color than Line C;
Please notice: Control line is used as a quality indicator, which will always appear regardless of the B/T line. If Control line does NOT appear, this indicates that the result is invalid. Users please check the kit insert again and re-do the assay with new test strips.
- Specificity
The kit has no cross reactions with Melamine ,clenbuterol, ractopamine, salbutamol, melamine, trenbolone, sulfonamides, chloramphenicol, macrolides and other drugs.
- Storage
2-8ºC in cool dark place, do not freeze. The kit will be valid in 12 months. The lot number and expired date are printed on the package.
- Notice for operations
- Please do the assay following the instruction, do not touch the membrane of the strip.
- Please seal the bottle after taking out required strips.
- This strip is used for only once; please do not use it repeatedly.
- This kit is only for screening test, positive result should be further confirmed with other method.
Detailed Photos
![Milk Test Strip Ilvo Bt 2 in 1](//www.micstatic.com/athena/img/transparent.png)
Company Profile
![Milk Test Strip Ilvo Bt 2 in 1](//www.micstatic.com/athena/img/transparent.png)
![Milk Test Strip Ilvo Bt 2 in 1](//www.micstatic.com/athena/img/transparent.png)
![Milk Test Strip Ilvo Bt 2 in 1](//www.micstatic.com/athena/img/transparent.png)
Stuff
Kwinbon currently has more than 500 employees, including 18 doctors, 92 masters and 24 senior engineers. And also 46% of the employees are working on R&D.
Stock Market
NEEQ (National Equities Exchange and Quotations); Stock Code: 835044.
Facility
GMP (Good Manufacturing Practice) factory and SPF animal house. The cleanliness of producing department can reach above level 105.
Instruments
HPLC, GC, LC-MS/MS for test result calibration, which are expected to provide better quality control of our test products.
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