Immunoaffinity Columns for Aflatoxin B1 for Food and Feed

• Express: DHL FedEx
• Samples: Rice Peanut Feed
• Recovery Rate: ≥80%
• Transport Package: Ice Bag with Foam Box
• Specification: 50T
• Trademark: kwinbon
• Model NO.: KH00102Z
• Origin: China
• HS Code: 38229000
• Production Capacity: 5000000000 PCS

Product Description

.Aflatoxin B1 Immunoaffinity columns

1. Principle

The aflatoxin B1 immunoaffinity column is based on antigen- antibody-reaction technology, which makes the test highly specific, sensitive, rapid and simple to perform.
The columns contain a gel suspension of monoclonal antibody specific to the toxin of interest. Following extraction of the toxin the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxin is then released from the column following elution with solvent. The final elution solution is for assay.
   It can reduce efficiently the sample impurity interference and improve the accuracy of test if it is combined with the HPLC, fluorescence and immunoassay.

1. Sample

It can test the maize, soybean, peanut, wheat, barley, rice and finished feed.

1. Required equipment

----Homogenizer
----Vortex mixer
----Sample bottle
----Measuring cylinder: 10ml, 100ml
----Qualitative filter paper/Centrifuge
----Analytical balance (inductance: 0.01g)
----Graduated pipette: 10ml
----Injector: 10ml
----Volumetric flask: 250ml
----Rubber pipette bulb
----Micropipette: 100-1000ul

1. Reagent

----Methanol (AR)
----Ethyl alcohol (AR)
----Hydrochloric acid (HCl, AR)
----Acetic acid (AR)
----Sodium chloride (NaCl, AR)
----Sodium acetate (AR)
----Trismetyl aminomethane (Tris, AR)
----Deionized water

1. Reagents preparation

Solution 1: 60% Methanol solution
Mix the deionized water and the methanol in the volume of 4:6. It is used for preparing the sample.
Solution 2: A solution
Take 3.03g trismetyl aminomethane and 7.31g NaCl and add 200ml deionized water. Mix completely. Regulate the PH value with HCl to 8.5±0.1 and finally the volume is 250ml.
Solution 3: B solution
Take 2.05g sodium acetate and 7.31g sodium chloride and add 200ml of deionized water. Mix completely. Regulate the PH value with acetic acid to 4.5±0.1 and the final volume is 250ml.
Solution 4: Preservation solution (20% ethyl alcohol)
Mix the deionized water and ethyl alcohol in the volume of 4:1.

1. Sample preparations

----Homogenize the sample.
----Take 5.0±0.05g sample into the sample bottle. Add 1.0±0.05g NaCl and 25ml of 60% methanol solution (solution 1) and vortex for 5min or shake for 20min with table concentrator. ----Centrifuge: 5min/3000g at least/room temperature (20-25ºC).(If no centrifuge, it can also stand the solution and take 10ml of the supernatant to filter)
----Take 5ml (similar to 1g sample) of supernatant after centrifuge or filter and add 5ml of deionized water. Mix completely and be for assay.

1. Assay process

----Take out the columns in the 2-8ºC/35.6-46.4ºF and recover to room temperature (20-25ºC/68-77ºF).
----Connect the columns to the 10ml injector by the joint (picture 1). Wash the column with 5ml deionized water for twice time.
----Pipette 5ml (0.5g sample) of prepared solution into the injector→→use the injector to control the flow rate of the liquid to 1-2 seconds per drop through the column.
---- Wash the column with 5ml deionized water for twice time →→use the injector to control the flow rate of the liquid to 0.5-1 seconds per drop through the column. Finally drain the liquid in the column.
----Elute with 1ml methanol→→use the injector to control the flow rate of the liquid to 2-3 seconds per drop through the column. Collect all the eluent and be for assay.
Notice:
----Peristaltic pump can also be used to control the flow rate to through the column.

• ----For a mass of test, using the pump flow operation can reduce the working time and intension.

8. Sample dilute ratio and recovery rate

Sample dilute ratio: 2 time

Recovery rate: ≥85%

9.Storage

Storage at 2-8ºC/35.6-46.4ºF. The period of validity is 12months.